Human stem cells from the dental pulp of adults (given by S. Gronthos, NIH, Bethesda, USA) were cultivated in a -modifi ed Eagle’s Medium (MEM) by Gibco BRL Life Technologies (Paisley, Scotland) while adding 20 % FCS, 2 mM L-glutamine, 100 μM L-ascorbate-2-phosphate, 100 u Penicillin and 100 μg/ml Streptomycin at 5 % CO 2 and 37°C in 25 ml and 75 ml cell culture fl asks (Greiner, Frickenhausen, Germany). 1 The cells were treated for experiments and cultivation with 0.25 % trypsin, 5 mM glucose, 0.05% EDTA in PBS for 5 minutes at 37 °C
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